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| Mon | May 6 | RE | Talbotville Optimist Park | 18:30 | Talbotville Womens S.C. | | Taxandria S.C. C |
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| Mon | May 6 | P | Windermere 1 | 20:15 | Alliance FC Galaxy | | Alliance FC Lasers |
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| Tue | May 7 | 2W | Henk Van Dyk Stadium | 20:30 | Strathroy United FC | | German Canadian S.C. Phoenix |
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| Tue | May 7 | 2W | Meadowcreek Upper | 18:30 | Ilderton District S.C. Ignite | | West End Blues S.C. |
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| Tue | May 7 | P | N. London 1 | 20:30 | London United Gryphons A | | St Thomas S.C. |
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| Tue | May 7 | 3 | Taxandria Soccer Complex #3 | 19:00 | Taxandria S.C. Taxandria B | | Taxandria S.C. Nairn |
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| Tue | May 7 | 2W | West Lorne Arena | 18:45 | Strike Force West Lorne Shooting Stars | | Sarnia Girls S.C. Spirit |
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| Wed | May 8 | 1 | Byron Lower | 21:00 | Byron Womens SC Fury | | Chatham YSC Chatham FC |
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| Wed | May 8 | P | N. London 2 | 20:15 | Alliance FC Lasers | | Alliance FC Galaxy |
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| Wed | May 8 | RE | West Lorne Arena | 18:30 | West Lorne Strikeforce | | Greater London S.C. Blizzard |
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| Thu | May 9 | RE | Coldstream Soccer #2 | 19:00 | Byron Womens S.C. Coldstream | | Glencoe Gals |
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| Thu | May 9 | 2W | East Williams - Nairn | 19:00 | Taxandria S.C. Nairn | | Southend United S.C. Fusion Black |
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| Thu | May 9 | 3 | Greenway 3 | 18:30 | Southwest Storm | | Wallaceburg S.A. Lady Sting |
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| Thu | May 9 | P | Marconi Club | 20:30 | Marconi Azzurri | | German Canadian FC Bullets |
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| Thu | May 9 | 2E | N. London 1 | 20:30 | London United S.C. Thunder | | St Marys S.C. Chargers |
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| Thu | May 9 | 2E | Portuguese Club | 18:30 | Portuguese Club Of London Thunder | | South Huron Rush |
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| Thu | May 9 | 3 | St. Columban CS | 18:30 | St. Columban S.C. | | London St Thomas Croatia |
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| Fri | May 10 | RE | Belmont CC 1 | 18:30 | Belmont S.C. A | | Belmont S.C. Thunder |
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| Sun | May 12 | 1 | German Canadian FC Stadium Field | 20:00 | German Canadian FC Fusion | | German Canadian FC Blaze |
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| Sun | May 12 | 2E | Goderich DCI | 18:15 | North Huron S.C. Tempest | | Portuguese Club Of London Thunder |
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| Sun | May 12 | 3 | Windermere 1 | 20:30 | Byron Womens S.C. Force | | Dorchester S.C. Sapphires |
